… Another highly specific and sensitive molecular method is nested RT-PCR (nRT-PCR)
which involves 2 rounds of PCR amplifications. As previously reported, the lowest …

… Compared with the previously established multiplex nested RT-PCR assay for the detection
of AIV, IBV, and Newcastle disease virus, our method is 5.2 times more sensitive for the …

… used in detecting IBV include nested PCR [68]; multiplex PCR [86… metapneumovirus (aMPV),
and avian influenza (AI), may … identification of avian infectious bronchitis virus by RT-PCR of …

… obtained from avian influenza and Newcastle disease-free … Nested PCR was performed in
a mixture of 20 μL containing … RTPCR assay for the detection of infectious bronchitis virus from …

… The avian influenza virus (AIV), Newcastle disease virus (NDV), and infectious bronchitis virus
… Many studies have also been performed using multiplex real-time RT-PCR to differentiate …

… chain reaction (RT-PCR) and its variants (multiplex, nested, … , are routinely used for detection
and genotyping of IBV strains [… , avian infectious laryngotracheitis virus, avian influenza virus…

… were frequently detected, with distinctive co-infection patterns … avian metapneumovirus
(aMPV), avian influenza virus (M gene… Potential utility of multiplex amplification respiratory viral …

… for the first time when avian influenza broke out in Asia in … RT-PCR, nested RT-PCR (nRT-PCR),
quantitative RT-PCR (qRT-… tools for detecting NDV and have the potential for a multiplex …

… RT-PCR may confirm the involvements of other respiratory pathogens in chickens as Newcastle
disease, avian influenza … -time RT-PCR assays were described that can detect IBV either …

… , 40 were class I Newcastle disease virus (NDV or APMV-1) … avian influenza viruses (AIVs),
one infectious bronchitis virus… of semi-nested or nested RT-PCR assays targeting the viral L …